Journal: Cell Communication and Signaling : CCS

Article Title: PPARγ agonism ameliorates acute kidney injury by inhibiting neutrophil extracellular trap formation-mediated renal tubular epithelial cell PANoptosis

doi: 10.1186/s12964-026-02686-6

Figure Lengend Snippet: PANoptosis occurs in the kidneys of mice treated with CP for 3 d. A Establishment of a CP-AKI mouse model. B Representative macroscopic images of kidneys and H&E-stained kidney sections from mice treated with CP for 3 d. Scale bar, 50 μm. C Cr levels measured on day 3 post-treatment. D BUN levels assessed on day 3 post-treatment. E Protein expression of MLKL, phosphorylated MLKL (p-MLKL), GSDMD, cleaved GSDMD (GSDMD-N), Caspase-3, and cleaved Caspase-3 (Cl-Cas3) in kidney tissues detected using western blot analysis. F Immunohistochemical staining for p-MLKL, GSDMD-N, and Cl-Caspase3 in kidney sections from CP-treated mice on day 3. Positively stained areas were quantified using Image-Pro Plus 6.0 (Media Cybernetics, Inc., Rockville, MD, USA). Scale bar, 50 μm. Data are presented as mean ± SEM of 3–6 biological replicates per condition, with 6 mice per group. Each dot represents a single sample. Statistical significance was assessed using an independent sample t-test (C–F). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Positively stained areas were quantified using Image-Pro Plus 6.0 software (Media Cybernetics, Inc., Rockville, MD, USA).

Techniques: Staining, Expressing, Western Blot, Immunohistochemical staining

Journal: Cell Communication and Signaling : CCS

Article Title: PPARγ agonism ameliorates acute kidney injury by inhibiting neutrophil extracellular trap formation-mediated renal tubular epithelial cell PANoptosis

doi: 10.1186/s12964-026-02686-6

Figure Lengend Snippet: In vivo administration of the NET inhibitor DNase I mitigates CP-induced renal injury and PANoptosis. A Schematic representation of DNase I injection in the experimental mouse model. DNase I (10 U/100 μL, dissolved in 0.9% NaCl) was administered daily for 3 d, consecutively, prior to CP treatment. B Representative macroscopic kidney images and H&E staining of kidney sections from CP-treated mice with or without DNase I co-treatment. Scale bar: 50 μm. C Cr levels measured in CP-treated mice co-treated with DNase I. D BUN levels measured in CP-treated mice co-treated with DNase I. E Renal mRNA expression levels of kidney injury molecule-1 (KIM-1) analyzed using real-time PCR. F Protein expression levels of MLKL, p-MLKL, GSDMD, GSDMD-N, Caspase-3, and cleaved Caspase-3 (Cl-Cas3) in kidney tissues evaluated using western blotting. G Immunohistochemical staining for p-MLKL, GSDMD-N, and Cleaved caspase-3 in kidney sections from CP-treated mice co-treated with DNase I. Positively stained areas were quantified using Image-Pro Plus 6.0 software (Media Cybernetics, Inc., Rockville, MD, USA). Scale bar, 50 μm. All data are expressed as mean ± SEM, with 3–6 biological replicates per condition and 6 mice per group. Each dot represents a single sample. Statistical significance was determined using independent sample t-tests or one-way ANOVA, followed by Tukey’s post hoc test ( C – F ). Statistical significance is indicated as follows: * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001

Article Snippet: Positively stained areas were quantified using Image-Pro Plus 6.0 software (Media Cybernetics, Inc., Rockville, MD, USA).

Techniques: In Vivo, Injection, Staining, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Immunohistochemical staining, Software